Journal: Journal of neuroscience methods
Article Title: An open-source method to analyze optokinetic reflex responses in larval zebrafish
doi: 10.1016/j.jneumeth.2017.10.012
Figure Lengend Snippet: OKR responses following unilateral stimulus presentation to the left eye were analyzed in 20 zebrafish larvae at 7dpf, using OKRtrack and OKRanalyze. Quantitative data are shown for 28 different parameters relating to movements of the stimulated (left) and contralateral (right) eyes, in response to a 15°/cycle, 99% contrast, sinusoidal grating pattern that moved at 15°/s, in a temporonasal (TN) or nasotemporal (NT) direction with respect to the left eye. Graphs show data points for individual larvae along with the group mean and 95% CI for A: ocular range; B: reflex gain; C: interocular difference; D: interocular concordance; E: interocular gain; F: saccade frequency; G: saccade velocity; H: saccade amplitude; J: saccade time constant. *p<0.05*, **p<0.01, ***p,0.001, ****p<0.0001, paired 2-tailed t-test (panels A, C, D, E) or 1-way ANOVA with Tukey’s post hoc test (panels B, F, G, H, J).
Article Snippet: New Method We developed an open-source MATLAB-based solution for automated quantitative analysis of OKR responses in larval zebrafish.
Techniques: